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multiscript high-capacity reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscript high-capacity reverse transcriptase
    Multiscript High Capacity Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscript+reverse+transcriptase/multiscript+high+capacity+reverse+transcriptase/pm39981650-138-8-13
    Average 90 stars, based on 1 article reviews
    multiscript high-capacity reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Transplacental arsenic exposure produced 5-methylcytosine methylation changes and aberrant microRNA expressions in livers of male fetal mice
    Article Snippet: .. Total RNA was reverse transcribed with Multiscript reverse transcriptase using a High Capacity RT kit from Applied Biosystems (Applied Biosystems, Foster City, CA). ..

    Article Title: Screening of Natural Compounds as Activators of the Keap1-Nrf2 Pathway
    Article Snippet: Twenty-four hours after treatment, cells were lysed in Trizol reagent (Life Technologies, Grand Island, NY) for total RNA isolation. .. Total RNA was reverse-transcribed into first-strand cDNA using multiscript reverse transcriptase from a High Capacity RT kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol. .. Messenger RNA of genes of Nqo1 was determined with quantitative real time-PCR performed on an Applied Biosystems Prism 7900HT sequence detection system.

    Article Title: Identification of 10 genes on Candida albicans chromosome 5 that control surface exposure of the immunogenic cell wall epitope β-glucan and cell wall remodeling in caspofungin-adapted mutants.
    Article Snippet: .. A total of 2 μg of total RNA was treated with DNase I (Thermo Fisher Scientific, MA, USA) followed cDNA preparation using multiscript reverse transcriptase (Applied Biosystems, MA, USA) as per manufacturer’s instruction. .. The quantitative amplification of cDNA was monitored by incorporation of SYBR green in StepOnePlus (Applied Biosystems, MA, USA) real-time qPCR system using PCR Master Mix (Applied Biosystems, MA, USA).

    Article Title: Potency of Individual Bile Acids to Regulate Bile Acid Synthesis and Transport Genes in Primary Human Hepatocyte Cultures
    Article Snippet: .. Total RNA was purified with RNeasy columns (Qiagen, Valencia, CA) and reverse transcribed with Multiscript reverse transcriptase using a High Capacity RT kit from Applied Biosystems (Foster City, CA). ..

    Article Title: Oeanolic acid protects against the hepatotoxicity of D-galactosame plus endotoxin in mice.
    Article Snippet: Oleanolic acid (OA) is a triterpenoid contained in many herbal medicines.. The aim of this study was to investigate the protective effect of OA against D-galactosame plus lipopolysaccharides (D-GalN/LPS)induced acute liver injury and the underling mechanisms.. Mice were randomly divided into normal control with vehicles only (corn oil), D-GalN/LPS only (700 mg/10 mg/kg, ip), OA-po (200 mmol/kg in corn oil, po) plus D-GalN/LPS, and OA-sc (50 mmol/kg in 2% tween 80, sc) plus D-GalN/LPS groups.

    Article Title: Hyperglycemia Increases the Production of Amyloid Beta‐Peptide Leading to Decreased Endothelial Tight Junction
    Article Snippet: Real‐Time Reverse Transcription–Polymerase Chain Reaction (RT‐PCR) Total RNA extraction was carried out using TRIzol reagent (Invitrogen) and RNA Clean & Concentrator TM ‐5 kit (Zymo Research, Irvine, CA, USA) according to the manufacturer's instructions. .. Following extraction, 0.5 μ g total RNA was reverse transcribed into cDNA with Multiscript reverse transcriptase using random primer and High Capacity cDNA Reverse Transcription kits (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Candida albicans Genes Modulating Echinocandin Susceptibility of Caspofungin-Adapted Mutants Are Constitutively Expressed in Clinical Isolates with Intermediate or Full Resistance to Echinocandins
    Article Snippet: .. A total of 2 μg of total RNA was treated with DNase I (Thermo Fisher Scientific, Waltham, MA, USA) followed by cDNA preparation using multiscript reverse transcriptase (Applied Biosystems, Bedford, MA, USA) as per manufacturer’s instruction. .. The quantitative amplification of cDNA was monitored by incorporation of SYBR green in the StepOnePlus qPCR system (Applied Biosystems, MA, USA) using a PCR master mix from Applied Biosystems (MA, USA).

    Purification:

    Article Title: Potency of Individual Bile Acids to Regulate Bile Acid Synthesis and Transport Genes in Primary Human Hepatocyte Cultures
    Article Snippet: .. Total RNA was purified with RNeasy columns (Qiagen, Valencia, CA) and reverse transcribed with Multiscript reverse transcriptase using a High Capacity RT kit from Applied Biosystems (Foster City, CA). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Oeanolic acid protects against the hepatotoxicity of D-galactosame plus endotoxin in mice.
    Article Snippet: Oleanolic acid (OA) is a triterpenoid contained in many herbal medicines.. The aim of this study was to investigate the protective effect of OA against D-galactosame plus lipopolysaccharides (D-GalN/LPS)induced acute liver injury and the underling mechanisms.. Mice were randomly divided into normal control with vehicles only (corn oil), D-GalN/LPS only (700 mg/10 mg/kg, ip), OA-po (200 mmol/kg in corn oil, po) plus D-GalN/LPS, and OA-sc (50 mmol/kg in 2% tween 80, sc) plus D-GalN/LPS groups.

    Extraction:

    Article Title: Hyperglycemia Increases the Production of Amyloid Beta‐Peptide Leading to Decreased Endothelial Tight Junction
    Article Snippet: Real‐Time Reverse Transcription–Polymerase Chain Reaction (RT‐PCR) Total RNA extraction was carried out using TRIzol reagent (Invitrogen) and RNA Clean & Concentrator TM ‐5 kit (Zymo Research, Irvine, CA, USA) according to the manufacturer's instructions. .. Following extraction, 0.5 μ g total RNA was reverse transcribed into cDNA with Multiscript reverse transcriptase using random primer and High Capacity cDNA Reverse Transcription kits (Applied Biosystems, Foster City, CA, USA). ..



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